Mammalian Cell Seeding
Enter your measured cell concentration and target cells per well to get a clear seeding plan: per-well pipetting volumes, total plate mix volume, and dilution guidance.
Preset loaded
cells/mL
%
cells/well
Additional substances (per well)
Add any extra components (e.g., antibiotic, nutrient, drug/compound). We calculate required volume per well and total volume for the full plate.
Per-well pipetting plan
Add 50 µL cell suspension + 0 µL additives + 450 µL base media per well.
Whole-plate mix plan
Target mix concentration: 100,000 cells/mL
Total dispense volume: 12,000 µL
Total additives volume: 0 µL
Prepare cell+base-media mix: 13,200 µL (13.2 mL)
Mix 1.32 mL cell stock + 11.88 mL media (about 1:10 dilution).