PCR Mastermix
Enter per-reaction volumes (µL). We’ll multiply by number of reactions + extra percent to avoid running short.
Multiplier: 11 reactions equivalent
| Reagent | Per reaction (µL) | Include in master mix? | Total (µL) | |
|---|---|---|---|---|
| Yes | 137.5 | |||
| Yes | 137.5 | |||
| Yes | 11 | |||
| Yes | 11 | |||
| Yes | 11 |
Total master mix volume: 297 µL (excluding items unchecked)
Total volume including everything: 308 µL
Tip: Many protocols add template separately (uncheck “include in master mix”).