Serial Dilution
Calculates stepwise concentrations and pipetting volumes for a serial dilution series.
e.g., 10 = 1:10, 2 = 1:2
Pipetting per tube
- Transfer: 100 µL
- Add diluent: 900 µL
Concentrations
| Tube | Concentration |
|---|---|
| 1 | 1 mM |
| 2 | 0.1 mM |
| 3 | 0.01 mM |
| 4 | 0.001 mM |
| 5 | 0.0001 mM |
| 6 | 0.00001 mM |
Transfer = final volume / dilution factor. Adjust factor/volumes to fit pipetting range.